Normal and CF matched cell pairs are co-transfected with either a plasmid coding for Firefly luciferase expression driven by a Nrf-2 or Nrf-1 promoter and one coding for Renilla luciferase driven by the CMV promoter. Panel A: Western analysis of nuclear (N) or whole cell (W) Nrf-2 and an anti-β actin control. Band densities are used to calculate the relative abundance chart. Panel B: Cells transfected with the Nrf-2 promoter luciferase construct. Panel C: Cells transfected with the Nrf-1 promoter luciferase construct. Two days following transfection cell homogenates were assayed for luciferase activity by luminometer. Unstimulated cells are compared to cells stimulated with TNFα/IL-1β (10 ng/ml each) alone, or in the presence of an activator of Nrf-2 but not Nrf-1, tBHQ. * connotes significant difference (p
3in1 Activator Shank
Our data are supported by previous studies [21], [22]. Our observation for GST and PRDX agree with pervious reports on CF epithelial proteomes [21], [22]. Increases in steady state H2O2 levels are intriguing since this has been shown to be a potent activator of NF-κB [16], [23], and may be the chief mediator of IL-1β receptor signaling in CF epithelia [10]. An imbalance in the intracellular H2O2 would increase redox mediated activation of NF-κB, and contribute to the excessive production of IL-6 and IL-8, which is present in the CF lung and which we observe in our cultured cell models.
Note: Aerosol Activator uses a solvent that affects plastics. Using too much activator may cause CA glue to ''boil,'' reducing the bond strength or creating unwanted whitening of the CA. Do not mix other brands of activator with this product; they may not be compatible. 2ff7e9595c
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